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NHS-dPEG™4-S-S-Biotin A Cleavable Biotin |
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Product Name: |
NHS-dPEG™4-S-S-Biotin |
| Product Number: | 10194 |
Quantities and Pricing
| Quantity(mg) | Price (USD) |
|---|---|
| 50 mg | $175.00 |
| 1000 mg | $875.00 |
Attributes
| Reactivity 1 | amine |
| Solubility | Methylene chloride or DMAC and DMSO |
| Molecular Weight | 751.94 |
| Special Notes | •Amine reactive biotinylation reagent •Disulfide cleavable; replaces an inferior sulfo-NHS-S-S-biotin •Very water soluble, hydrophilic and eliminates non-specific binding •Ideal spacer length for binding streptavidin conjugates; chain length from amide to terminal carbonyl is 28.7 Angstroms and 24 atoms •dPEG™ linker is designed to be the same length as the LC-LC spacer, which is ideal for the streptavidin binding pocket •Non-antigenic and non-immunogenic spacer arm •ELIMINATES aggregation and precipitation when labeling antibodies and other biological materials (a severe problem with conventional biotinylation reagents) |
Protocols
| Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). Use protocol on page 377, except make a 40 mg/ml solution of the NHS-biotin in dry DMF. The use of dry DMF is critical. This can easily be obtained by placing 3 Angstrom or 4 Angstrom molecular sieves into reagent grade DMF, shaking and letting stand at least overnight. If possible handle the NHS-biotin under an inert atmosphere. We find that handling these materials in an Atmosbag, available from Sigma-Aldrich, under argon (preferred) or nitrogen, is the best, most convenient and inexpensive means of handling these moisture sensitive materials. The remaining solid NHS-biotin should be stored under an inert gas (argon preferred) at about 4 Celsius. The product can be pre-dissolved in an organic solvent OR can be directly dissolved in your reaction medium based on its inherent water solubility. |
| Spacer length (same as LC-LC spacer) is optimal for rapid and tight avidin/streptavidin binding. Hydrophilic spacer arm eliminates non-specific binding that causes aggregation and precipitation problems, which commonly occur when labeling antibodies and other biological materials. Hydrophilic dPEG™ spacer is non-immunogenic. Ultra high purity. |
References
| Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). See pages 377-380. |
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