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 NHS-LC-Biotin
| Product Name: |
NHS-LC-Biotin |
| QBD Product Number: |
10206 |
| Category |
Conventional Biotinylation Reagents |
Quantities and Pricing
| Quantity |
Price (USD) |
| 50 mg |
$80.00 |
| 1000 mg |
$650.00 |
| |
|
| Bulk |
Please call or e-mail for pricing. |
Attributes
| Reactivity 1 |
amine |
| Solubility |
requires use of DMF, but more soluble than NHS biotin |
| Spacer length (A) |
8.8 |
| Molecular Weight |
454.54 |
| Special Notes |
Spacer arm to enhances binding with streptavidin. |
Protocols
| Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). See pages 379-380. |
| Hermanson, Greg T, Nucleic Acid & Oligonucleotide Modification & Conjugation: Biotin Labeling of DNA. Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). See page 658 for commentary regarding biotinylation of diamine-modified DNA probes. |
References
| Bayer, E.A., Ben-Hur, H., and Wilchek, M. Colorimetric Enzyme Assays for Avidin and Biotin. Avidin-Biotin Technology: Methods in Enzymology, Vol. 184, M. Wilchek and E. A. Bayer, eds., Academic Press, Inc. San Diego, CA. 1990. See pages 217-223. |
| Meier, K.E. and Ruoho, A.E. Probing B-Andrenergic Receptors. Avidin-Biotin Technology: Methods in Enzymology, Vol. 184, M. Wilchek and E. A. Bayer, eds., Academic Press, Inc. San Diego, CA. 1990. See pages 660-668 for commentary regarding small molecule biotinylated agonists. |
| Hermanson, Greg T, Antibody Modification & Conjugation: Preparation of Labeled Antibodies-Biotinylated Antibodies. Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). See page 492. |
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