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NHS-biotin
| Product Name: | NHS-biotin |
| QBD Product Number: | 10205 |
| Category | Conventional Biotinylation Reagents |
Quantities and Pricing
| Quantity | Price (USD) |
|---|---|
| 100 mg | $65.00 |
| 1000 mg | $250.00 |
| Bulk | Please call or e-mail for pricing. |
Attributes
| Reactivity 1 | amine |
| Molecular Weight | 341.38 |
| Special Notes | Critical & versatile building block for many biotinylation reagents. Efficient label for certain peptides and nucleic acids. Permeates cell membranes. Quanta's exclusive synthetic process produces the highest quality, ultra pure material. |
Protocols
| Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). Use protocol on page 377, except make a 40 mg/ml solution of the NHS-biotin in dry DMF. The use of dry DMF is critical. This can easily be obtained by placing 3 Angstrom or 4 Angstrom molecular sieves into reagent grade DMF, shaking and letting stand at least overnight. If possible handle the NHS-biotin under an inert atmosphere. We find that handling these materials in an Atmosbag, available from Sigma-Aldrich, under argon (preferred) or nitrogen, is the best, most convenient and inexpensive means of handling these moisture sensitive materials. The remaining solid NHS-biotin should be stored under an inert gas (argon preferred) at about 4 Celsius. |
| Dutton, A.H., Adams, M., and Singer, S.J. Double Fluorescence Immunolabeling Studies. Avidin-Biotin Technology: Methods in Enzymology, Vol. 184, M. Wilchek and E. A. Bayer, eds., Academic Press, Inc. San Diego, CA. 1990. See pages 346-347 for commentary regarding Biotinylation of Mab. |
References
| Sutoh, K. Visualization of Adenotriphosphatase site of Myosin Using Photoreactive Adenosine 5'-Diphosphate Analog. Avidin-Biotin Technology: Methods in Enzymology, Vol. 184, M. Wilchek and E. A. Bayer, eds., Academic Press, Inc. San Diego, CA. 1990. See pages 418-419 for commentary regarding prelabeling of lysine amino groups in proteins. |
| Avidin-Biotin Technology: Methods in Enzymology, Vol. 184, M. Wilchek and E. A. Bayer, eds., Academic Press, Inc. San Diego, CA. 1990. |
| Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). |
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