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 NHS-dPEG4-Biotin
| Product Name: |
NHS-dPEG4TM-Biotin |
| QBD Product Number: |
10200 |
| Category |
Biotinylation Reagents with
Water Soluble, Non-Immunogenic
Discrete PEG (dPEGTM) Spacers |
Quantities and Pricing
| Quantity (mg) |
Price (USD) |
50 |
$100.00 |
| 1000 |
$750.00 |
| Bulk |
Please call for pricing. |
Attributes
| Reactivity 1 |
amine |
| Solubility |
highly water soluble |
| Spacer length (A) |
19.2 |
| Molecular Weight |
588.67 |
| Special Notes |
Spacer length (same as LC-LC spacer) is optimal for rapid and tight avidin/streptavidin binding. Hydrophilic spacer arm eliminates non-specific binding that causes aggregation and precipitation problems, which commonly occur when labeling antibodies and other biological materials. Hydrophilic dPEGTM spacer is non-immunogenic. Ultra high purity. |
Protocols
| Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). Use protocol on page 377, except make a 40 mg/ml solution of the NHS-biotin in dry DMF. The use of dry DMF is critical. This can easily be obtained by placing 3 Angstrom or 4 Angstrom molecular sieves into reagent grade DMF, shaking and letting stand at least overnight. If possible handle the NHS-biotin under an inert atmosphere. We find that handling these materials in an Atmosbag, available from Sigma-Aldrich, under argon (preferred) or nitrogen, is the best, most convenient and inexpensive means of handling these moisture sensitive materials. The remaining solid NHS-biotin should be stored under an inert gas (argon preferred) at about 4 Celsius. The product can be pre-dissolved in an organic solvent OR can be directly dissolved in your reaction medium based on its inherent water solubility . |
References
| Hermanson, Greg T, Bioconjugate Techniques, Academic Press, Inc., San Diego, CA, 1996 (ISBN 0-12-342335-X). See pages 377-380. |
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